Molecular Biology Reagents and Kits
Norgen Biotek Corp RNase-Free DNase I Kit, 200 Reactions
Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.
Norgen Biotek Corp Fungi/Yeast Genomic DNA Isolation Kit
Norgen's Fungi/Yeast Genomic DNA Isolation kit provides rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and Gram-positive and Gram-negative bacteria. Genomic DNA is efficiently extracted from the cells by a combination of heat treatment, detergents and bead tubes (provided). An optional lyticase treatment allows for improved DNA yields with certain fungal and yeast species. Protocol time is typically less than 30 minutes. Recovered genomic DNA is of excellent yield and purity for any downstream application including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), sequencing, SNP analysis and more.
Beckman Coulter Reagent, SPRIselect, 450mL
Used for Purification & Clean-up, Nucleic acid sample prep, DNA Size Selection, RNA Size Selection, NGS Clean-up, PCR clean-up, including size selection
Zymo Research Corporation Quick-DNA/RNA Viral MagBead (4x 96 preps)
The Quick-DNA/RNA™ Viral MagBead kit also features a buffer system that facilitates complete viral particle lysis for efficient nucleic acid isolation. Small (> 50 nt) and large (> 200 kb) DNA and RNA are bound to magnetic beads, washed and eluted.The isolated high-quality nucleic acids are ready for all downstream applications such as Next-Gen sequencing, hybridization-based andRT/qPCR detection.
Agilent Technologies Agilent DNA 1000 Reagents
Agilent DNA 1000 Reagents. Reagents only; no chips included. Sufficient for 25 chips.
Norgen Biotek Corp Dna Isolation Kit Saliva
This kit provides a fast and simple spin column procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples.Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.
Zymo Research Corporation Select-a-Size DNA MagBead Kit
This simple workflow allows for specific cutoffs thatcan be modified to suit reaction clean-up, left-sided, right-sided or even double-sided size selection. The Select-a-Size MagBead Buffer selectively binds fragments based on the volume added relative to the sample. Simply choose a desired cutoff to bind the target of interest onto the beads and remove species outside of this range. The desired DNA is easily eluted from the beads following a rapid wash regimen.
Zymo Research Corporation Select-a-Size DNA MagBead Kit
This simple workflow allows for specific cutoffs thatcan be modified to suit reaction clean-up, left-sided, right-sided or even double-sided size selection. The Select-a-Size MagBead Buffer selectively binds fragments based on the volume added relative to the sample. Simply choose a desired cutoff to bind the target of interest onto the beads and remove species outside of this range. The desired DNA is easily eluted from the beads following a rapid wash regimen.
Norgen Biotek Corp microRNA (cel-miR-39) Spike-In Kit
Norgen’s microRNA (cel-miR-39) Spike-In Kit offers a quantified synthetic RNA (cel-miR-39) for spike-in during RNA extraction and subsequent normalization in RT-qPCR assays. The amount of cel-miR-39 RNA recovered after RNA extraction is directly correlated with the amount of total RNA recovered. After reverse transcription of the sample RNA (with spike-in), the level of cel-miR-39 can be determined by subjecting the cDNA generated to quantitative PCR using fluorescent nucleic acid stains such as SYBR Green. A cel-miR-39 specific primer is included in the kit. The level of expression of any target transcripts in different RNA samples can be normalized to the cel-miR-39 transcript level using standard methods such as ∆∆Ct relative quantification.cel-miR-39 RNA is compatible with library preparation methods in Small RNA-Seq workflows. cel-miR-39 RNA can be used for normalization and tracking library construction efficiency.